Invitrogen’s Expressway™ NMR Cell-Free E. coli Expression System was specifically designed for NMR protein structure analysis. The enhanced in vitro transcription/translation kit is able to generate milligram-quantities of soluble proteins within 6 hours and non-natural amino acids containing 13C, 15N are incorporated into newly synthesized proteins with close to 100% efficiency. In some cases, since the label is incorporated only into newly synthesized proteins, it is possible to perform NMR analysis without prior protein purification. The small reaction volume also reduces the cost of non-natural amino acid incorporation. This system is one of the fastest, easiest and most cost-effective tools for making proteins for NMR analysis. For details, please visit www.invitrogen.com/expressway.
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